Cosmetics & Dermatology
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With the technological advancements in cell culture, the industry is moving towards cruelty-free research, wherein animals are no longer used for cosmetic development and testing. While regulatory authorities are also not in favor of animal testing in the first instance, the global cosmetic formulation is slowly shifting towards primary cells isolated from human tissues; as cardinal models for cosmetic testing.
- Primary Human Skin Fibroblasts
- Primary Human Keratinocytes
- Primary Human Melanocytes
- Primary Human Vaginal Epithelial Cells
- Primary Human Skin Epithelial Cells
- Balb/c 3T3 Mouse Fibroblast cell line
- 脂肪Tissue derived exosomes
- Skin Tissue derived exosomes
- Complete growth medium for fibroblasts
- Complete growth medium for keratinocytes/melanocytes
The dermal phototoxicity of active ingredients in cosmeceutical products is defined as the toxic response, elicited after exposure to primary skin models with the product. Several validation studies have demonstrated how the phototoxic potential of active ingredients can be assessed using various in vitro methods.
If a particular active ingredient is absorbing more UV light or visible light, it is very important to assess, whether this extensive absorption is causing any toxic effects when intended for human use. So far, various studies have been performed using immortalized mouse fibroblast cell lines Balb/c 3T3. However, using certain primary cells like keratinocytes, melanocytes, etc. various skin models can be reconstructed at different stages of maturity. These models were found to be histologically similar to that of in vivo human epidermis. The models can be further studied for specific differentiation markers like loricrin, keratin 10, keratin 5, etc.; along with some dermal/epidermal skin markers, such as Type IV collagen, laminin V, etc.
Accordingly, various other models can be prepared, such as:
- Skin irritation model
- Photoprotection model
- Skin corrosion model